Determining Turbidity of an aqueous solution is a frequently used procedure to estimate the quality of water, or even concentration of suspended particles such as cells, bacteria and mud.Turbidity can be defined as a decreased in the transparency of a solution due to the presence of suspended and some dissolved substances, which causes incident light […]
Author Archives: Preston Ellapen
A method for on-site nickel measurements for the metal plating industry is introduced. By using the Photopette® handheld spectrophotometer [1, 2] only minimal training is requited. The method is easy and fast performed in two steps 1) Calibration and 2) Measurement. The calibration takes about 3 minutes and is stable for several days. A measurement […]
Spirulina is a microscopic and filamentous cyanobacterium. It is promoted as a ‘superfood’ that contribute to high energy levels. The important nutrients are polysaccharides and essential fats which are easily absorbed by human cells and help in energy release [1]. Generally, the growth of the bacteria is characterized by five stages. To maintain a healthy […]
The food & beverage industry must make many tests and take certain measurements to ensure that their products meet high standards and are safe for consumption. The Photopette can be used for photometric measurements of liquid samples. The objective of this application note is to demonstrate how Photopette Cell can be used to quantify the […]
E.coli cell counting at OD600 is demonstrated using the Photopette® handheld spectrophotometer [1, 2]. Almost no training is requited. The method is easy and fast performed at the bioreactor or in the cell culture hood. E. coli can be directly measured at 600 nm in the cell culture flask. The method can be performed at […]
Yeast cell counting at OD600 is demonstrated using the Photopette® handheld spectrophotometer [1, 2]. Almost no training is requited. The method is easy and fast performed at the brewery or in the cell culture hood. Yeast can be directly measured at 600 nm in the cell culture flask. The method can be performed at any […]
The objective of this application note is to demonstrate how Photopette Cell can be used to measure cell-viability of eukaryotic cells using a Resazurin Assay. Furthermore, it acts as a handy guide to get you started with Photopette Cell, and outlines application-specific parameters for reference. Cell-viability assays are important cell-based assays to determine whether cells […]
Here we demonstrate a method to measure the pantoprazolecontent in tablets within 10 minutes. There are no reagentsnecessary. By using the Photopette® handheldspectrophotometer [1, 2] only minimal training is requited.The method is easy and fast performed in two steps 1)Calibration and 2) Measurement. The method can beperformed at any location and does not require a […]
During anaerobic glycolysis, energy is produced. In this process, lactate dehydrogenase (LDH) catalyses the oxidation of lactate to pyruvate and an equimolar amount of nicotinamide adenine dinucleotide (NAD+) is reduced to its reduced form NADH. The amount of NADH produced is directly proportional to the lactate concentration in the sample [1]. The produced NADH is […]
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Through the years cell counting has become increasingly more important and central to many medical and research applications. This created a need for a more efficient method of cell counting. In response, NanoEntek created the Countess Automatic Cell Counter. This instrumented reduced the time and effort of cell counting. The Countess became a well known ODM product and sold more than 10,000 devices wordlwide. This effectively launced the Automatic Cell Counter market.