Tag Archives: Handheld spectrophotometers

Turbidity measurement of unknown liquid samples using Photopette

Determining Turbidity of an aqueous solution is a frequently used procedure to estimate the quality of water, or even concentration of suspended particles such as cells, bacteria and mud.Turbidity can be defined as a decreased in the transparency of a solution due to the presence of suspended and some dissolved substances, which causes incident light […]

Measuring Cell-Viability By Resazurin (AlamarBlue®) Assay Using Photopette® Cell

The objective of this application note is to demonstrate how Photopette Cell can be used to measure cell-viability of eukaryotic cells using a Resazurin Assay. Furthermore, it acts as a handy guide to get you started with Photopette Cell, and outlines application-specific parameters for reference. Cell-viability assays are important cell-based assays to determine whether cells […]

DETERMINATION OF LACTATE CONCENTRATION USING PHOTOPETTE CELL

During anaerobic glycolysis, energy is produced. In this process, lactate dehydrogenase (LDH) catalyses the oxidation of lactate to pyruvate and an equimolar amount of nicotinamide adenine dinucleotide (NAD+) is reduced to its reduced form NADH. The amount of NADH produced is directly proportional to the lactate concentration in the sample [1]. The produced NADH is […]

MEASURING PROTEIN CONCENTRATION DIRECTLY USING PHOTOPETTE® BIO

Download PDF version of this article here Measuring Protein Concentration Directly using Photopette® Bio Photopette® enables fast protein measurements directly at the bench.Protein samples can be directly measured at 280 nm without sample preparation and sample transfer. Objective: The objective of this application note is to demonstrate how the Photopette® Bio device can directly measure […]

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