The image analysis software from Azure Biosystems provides a comprehensive platform for accurate image analysis of Western blots, gels, plates, and arrays. Offered through Apex Scientific in South Africa, AzureSpot Pro streamlines complex workflows and delivers reproducible, publication-ready data for scientists using demanding laboratory equipment.
Integrated Analysis Tools
AzureSpot Pro combines several modules in one intuitive package. Therefore, researchers can perform multiplex and single-channel imaging, area and profile analysis, and colony counting with exceptional accuracy. In addition, automatic lane and band detection accelerates Western blot interpretation, while manual adjustments give users complete control.
Single-channel / Multi-channel Images
AzureSpot Pro simplifies analysis of in-cell Western blots, traditional Western blots, and other imaging workflows. Researchers can process single-channel or multichannel images with flexible viewing—display individual channels, overlay all channels at once, or remove selected channels for clarity. The software also streamlines data quality checks. Automatic saturation detection flags overexposed pixels, while an accompanying 3D image model reveals signal distribution and trends before detailed analysis begins. Native multiplex support provides an expanded range of pseudocolor options, enabling clear differentiation of multiple targets in a single experiment and ensuring accurate, publication-ready results.
Advanced Normalization and Calibration
Furthermore, grid-based normalization supports plate and array assays, enabling reliable presence-or-absence detection. Built-in molecular-weight and quantity-calibration tools simplify total protein normalization as well as multiplex Western blot quantification. Various background-subtraction methods, including rolling ball and image rectangle, further improve data precision.
Multiple Lan Boxes
Multiple lane boxes allow for simultaneous analysis of multiple blots, gels or plates in a single image. Customize the number of lanes or lane boxes, and even the shape of each box and lane for the most accurate analysis of each image.
Background Subtraction
Choose from several background subtraction methods, including rolling ball and image rectangle, with the ability to customize background subtraction for each channel within your image. Background subtraction allows for more accurate analysis in certain situations such as total protein detection and normalization.
Flexible Imaging Capabilities
The software also supports large files and multi-page TIFFs. With native multiplex imaging and customizable lane boxes, scientists can analyze multiple blots or gels simultaneously. In addition, a 3D saturation model highlights signal trends before detailed evaluation, ensuring high-quality results every time.
Band Detection
Band detection options allow users to customize and fine tune analysis to their unique analysis style. Use automatic band detection, detect bands manually using lane profiles, or detect by clicking on the image itself. Customize automatic band detection by channel or detect globally across a multichannel image with one set of parameters.
Multiple Analysis Modules
AzureSpot Pro delivers a comprehensive suite of analysis modules and robust file support for complex imaging tasks. Researchers can work with multiplex or single-channel gels and blots, perform plate and array evaluations, conduct precise area and profile analyses, and count colonies accurately. The software easily handles large image files with native multi-page TIFF compatibility, while the Array module’s grid-based system streamlines plate-based assays with built-in normalization and presence/absence detection, ensuring reliable and reproducible results across diverse laboratory applications.
Applications in Modern Research
Consequently, AzureSpot Pro suits chemiluminescent and fluorescent Western blots, antibody and membrane arrays, and bacterial colony imaging. Laboratories across South Africa can integrate this image analysis software into existing laboratory equipment setups for efficient data handling and interpretation.
Explore advanced imaging solutions and learn more on our Insights page. To download AzureSpot Pro directly, visit the Azure Biosystems website.
Applications
