CytoView MEA plate, the premium Maestro multiwell microelectrode array (MEA) plate, combines unparalleled access to cellular electrical network information with a thin, transparent well bottom for culture visualization and assay multiplexing. Available in 6-, 24-, 48-, and 96-well formats (see table below), CytoView MEA plates contain the industry-leading electrode count per well (up to 64 electrodes per well), deliver low-noise signals, and can be read over days, weeks, or months.
Key Features
Get more from every assay.
- Record high quality data – Industry-leading electrode count for detailed information from your cardiac and neural assays. PEDOT electrode technology ensures collection of the highest quality signals.
- See your cells with transparent plates – Compatible with light microscopy for daily culture monitoring.
- Assay multiplexing enriches MEA data – Multiplex your assay with top- or bottom-read fluorescent and luminescent plate readers. Choose between black or white walls for optimal application flexibility.
| CytoView MEA
The CytoView MEA plates combine robust data collection with a transparent well bottom for cell visualization and assay multiplexing |
||||||||||
| Plate | Cat No. | Wells | Electrode/ well |
Electrode layout* |
Bottom | Walls | Maestro Edge |
Maestro Pro |
Maestro Z/ZHT |
Maestro Original |
|---|---|---|---|---|---|---|---|---|---|---|
| CytoView MEA 6 | (a) M384-tMEA-6B
(b) M384-tMEA-6W |
6 |
64 PEDOT |
 |
Transparent | (a) Black (b) White |
● | ● | ● | |
| CytoView MEA 24 | M384-tMEA-24W |
24 |
16 PEDOT |
 |
Transparent | White | ● | ● | ||
| CytoView MEA 48 | (a) M768-tMEA-48B
(b) M768-tMEA-48W |
48 |
16 PEDOT |
 |
Transparent | (a) Black (b) White |
● | ● | ||
| CytoView MEA 96 | (a) M768-tMEA-96B
(b) M768-tMEA-96W |
96 |
8 PEDOT |
 |
Transparent | (a) Black (b) White |
● | ● | ||
*Schematic of well illustrating recording electrodes (blue), grounds (orange), and where present, a large dedicated stimulation (blue).
Overview
Cell visualization and assay multiplexing
The innovative, transparent plate bottom offers additional assay flexibility including cell visualization and assay multiplexing. Bright field imaging enables confirmation of cell spotting accuracy and correlation of cell culture health and connectivity with MEA results. Multiplex fluorescence- or luminescence-based assays with your MEA study to probe complementary end points.
High quality MEA data
Both cardiomyocytes and neurons perform well on the CytoView MEA plates, showing excellent coverage across all wells and the high signal-to-noise ratio Axion customers expect.
Assay multiplexing enriches MEA data
With the transparent CytoView MEA plates, reporter-based (ex. fluorescent or luminescent) assays can be used to complement MEA data generated from the same well. The combination of electrophysiological data with reporter-based assays can provide supplementary information regarding compound mechanism of action.
In this neurotoxicity assay, compound were assessed using MEA electrophysiology data from Axion’s Maestro MEA system multiplexed with a fluorescence lactate dehydrogenase (LDH) cell viability assay. Multiplexing enabled greater specificity for compound classification compared to MEA data alone. (A) Both deltamethrin and tributyltin reduced mean firing rate, but (B) only tributyltin reduced cell viability. (Data provided by external customer)
Related Applications
- Cardiac Classification – Predict the cardiomyocyte subtype using the action potential waveform in the LEAP assay
- Cardiac Development – Track cardiac differentiation and maturation over weeks and determine when cardiomyocytes start to beat and form a syncytium
- Cardiomyocyte Pacing – Control cardiomyocyte beat rate with optical or electrical stimulation to increase the physiological relevance of the assay
- Cardiotoxicity and Safety – Evaluate the proarrhythmic risk of compounds in acute and chronic exposure experiments in CiPA-style studies
- Inotropy – Characterize compound-induced effects on cardiomyocyte inotropy and excitation-contraction coupling
- Neural Characterization and Development – Monitor neural development and quantify network dynamics to identify unique properties of your culture
- Neural Circuit and Innervation – Track neural innervation and functional network formation between two discrete cell populations
- Neural Co-culture and Glial Interaction – A wide variety of neurons and other cells exist and interact together and the composition of a neural culture can alter development and disease progression.
- Neural Optical and Electrical Stimulation – Evoke neural activity with optical or electrical stimulation
- Neural Organoids – Measure neural activity from live neural organoids and characterize advanced network behavior
- Neurological Disease – Explore the role your gene of interest has on neural network activity in patient-derived iPSCs or animal models
- Neuromuscular Junction – Record evoked skeletal myotube activity in functional iPSC-derived neuromuscular junctions
- Neurotoxicology and Safety – Characterize the neuroactive properties of compounds in acute and chronic exposure experiments
- Pain – Measure modulations of DRG neuron activity in response to chemical or temperature stimuli
- Retina – Record light-evoked electrical activity from primary retina slices and iPSC-derived retinal organoids
- Zebrafish – Track neural activity, from zebrafish brain and spinal cord, label free
