The Neutrophil isolation kit by StemCell Technologies, available through Apex Scientific, South Africa’s leading laboratory equipment supplier, provides a fast and efficient way to isolate highly purified human neutrophils directly from whole blood samples. It uses an immunomagnetic negative selection method that targets unwanted cells for removal, leaving the neutrophils untouched. For over 20 years, research laboratories worldwide have relied on EasySep™ technology. This system combines the specificity of monoclonal antibodies with the convenience of a column-free magnetic method. As a result, scientists can obtain pure neutrophil populations without tedious density gradients or red blood cell lysis.
How It Works
The Neutrophil isolation kit employs a simple immunomagnetic negative selection process in which a cocktail of antibodies and magnetic particles (EasySep™ Direct RapidSpheres™) first labels the unwanted cells (including basophils, eosinophils, T cells, B cells, monocytes, NK cells, and erythroid cells). Next, place the sample into an EasySep™ magnet, which captures those labeled cells. This allows you to simply pour the untouched neutrophils into a fresh tube. Additionally, the procedure leaves the neutrophils untouched by any beads or antibodies, ensuring they remain unaltered and ready for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.
Moreover, this updated Neutrophil Isolation Kit replaces the earlier version (Catalog #19257) to deliver even faster cell isolation times. Furthermore, high-throughput laboratories can fully automate the process using the RoboSep™ instrument, which increases laboratory throughput and consistency.
Key Advantages
- High RBC Depletion: Removes over 99.9% of red blood cells without the need for density gradient centrifugation, sedimentation, or lysis steps.
- High Purity: Achieves up to 99% purity of neutrophils, yielding reliable results for sensitive downstream assays.
- Fast and Easy Protocol: Offers a quick, easy-to-use procedure that is column-free, saving valuable laboratory time.
- Untouched Cell Isolation: The isolation process leaves neutrophils free of any attached labels or beads, preserving their native state and function.
Kit Contents and Formats
The isolation kit is available in two formats to suit different laboratory workflows. Each kit comes with the necessary reagents and is supplied by StemCell Technologies, with local availability through Apex Scientific in South Africa:
- EasySep™ Direct Human Neutrophil Isolation Kit (Catalog #19666) – includes EasySep™ Direct Human Neutrophil Isolation Cocktail (2 × 2.5 mL) and EasySep™ Direct RapidSpheres™ (4 × 2.5 mL).
RoboSep™ Human Neutrophil Isolation Kit (Catalog #100-0404) – includes EasySep™ Direct Human Neutrophil Isolation Cocktail (2 × 2.5 mL), EasySep™ Direct RapidSpheres™ (4 × 2.5 mL), RoboSep™ Buffer (Cat #20104), and RoboSep™ Filter Tips (Cat #20125).
Magnet Compatibility
This is compatible with a range of EasySep™ magnets and instruments from StemCell Technologies, including:
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50™ EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S Instrument (Catalog #21000)
Applications
The direct human neutrophil isolation kit supports a variety of research areas in immunology and infectious diseases. For example, it enables scientists in clinical and academic laboratories to quickly obtain pure neutrophils for functional assays, immune response studies, or pathogen interaction experiments. By using this neutrophil isolation kit, laboratories in South Africa can improve the efficiency and consistency of neutrophil-based experiments, which is crucial for high-quality research outcomes.
For further insights and practical guidance, visit our Insights page, where Apex Scientific offers expert resources for laboratory professionals. Ultimately, Apex Scientific is committed to empowering scientists with high-quality laboratory equipment and support in South Africa.
