The Lymphocyte isolation kit by StemCell Technologies, available from Apex Scientific in South Africa, offers a fast, column-free method to isolate highly purified human lymphocytes. It works directly from whole blood, buffy coat, or spleen samples using immunomagnetic negative selection. With over two decades of use in published immunology research, this kit provides a trusted and efficient approach to lymphocyte isolation.
The EasySep™ Direct Human Total Lymphocyte Isolation Kit combines targeted monoclonal antibodies with magnetic bead separation. It eliminates the need for density gradients, centrifugation, or additional laboratory equipment. The process requires only a compatible magnet, simplifying your workflow significantly. Apex Scientific supports South African laboratories by supplying this advanced kit and offering local expertise to ensure successful implementation.
Immunomagnetic Separation – Simple and Effective
This kit uses negative selection to remove unwanted cells, leaving lymphocytes untouched. The antibody cocktail targets non-lymphocyte cells—monocytes, neutrophils, eosinophils, and platelets—and binds them to RapidSphere™ magnetic particles. After labeling, the sample goes into an EasySep™ magnet. The magnet pulls the labeled cells to the tube’s side. You can then pour off the untouched lymphocytes into a fresh tube. These cells are ready for applications like flow cytometry, culture, or DNA/RNA extraction without further purification.
Key Benefits for Your Laboratory
The Lymphocyte isolation kit offers multiple benefits:
- Up to 96% purity of total lymphocytes ensures accurate downstream results.
- Over 99.9% red blood cell removal without gradient centrifugation, sedimentation, or lysis.
- Fast, column-free workflow with minimal handling and no extra equipment.
- Untouched cells maintain functional integrity for sensitive assays.
- Automation-ready via RoboSep™ for high-throughput environments.
Formats and Compatibility
You can use this kit with manual EasySep™ magnets like the standard unit, “Big Easy” Magnet, or EasyEights™ for larger batches. It also integrates seamlessly with the RoboSep™ – S instrument for full automation. The RoboSep™ – compatible format (Catalog #19655RF) includes buffer and filter tips for hands-free operation. Both manual and automated formats include an antibody cocktail and RapidSpheres™, ready for immediate use.
Apex Scientific provides all supporting laboratory equipment—from magnets to RoboSep™ systems, ensuring compatibility across workflows. For expert advice and more tools to support your research, visit the Apex Scientific Insights page.
