The CD15 positive selection kit technology allows scientists to rapidly isolate highly purified human CD15+ cells from fresh whole blood or buffy coat samples. Using an immunomagnetic positive selection method, the EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit from StemCell Technologies provides a fast, column-free solution. Apex Scientific supplies this trusted laboratory equipment in South Africa, helping researchers streamline workflows and generate reliable results. The kit has been widely used for over 20 years in immunology and chimerism studies.
Key Advantages
- Fast and Easy-to-Use: Simplified protocol allows quick isolation of target cells with minimal hands-on steps, improving efficiency in the lab.
- High Purity (Up to ~99%): Achieve extremely pure CD15+ cell fractions – typically around 99% purity of the target cells – ensuring reliable results for sensitive applications.
- No Columns Required: The immunomagnetic system works without separation columns, reducing additional equipment needs and simplifying your laboratory setup.
How It Works
The kit uses monoclonal antibodies that recognize CD15, a marker found on granulocytes and monocytes. These antibodies bind with magnetic particles to label target cells. After incubation, users place the sample in an EasySep™ magnet. Unlabeled cells are removed by pipetting or pouring. The enriched CD15+ cells remain in the tube, untouched and ready for downstream use.
Because the process is gentle, cell viability remains high. Researchers can immediately proceed with flow cytometry, culture, or DNA/RNA extraction. This is especially useful in lineage-specific chimerism analysis, where purified myeloid cells are essential.
Manual and Automated Formats
You can use the standard kit (Catalog #17881) with EasySep™ magnets like “The Big Easy” (Catalog #18001) or EasyEights™ (Catalog #18103). Alternatively, choose the RoboSep™ – compatible version (Catalog #17881RF) for automated processing. This version includes RoboSep buffer and filter tips, making it ideal for high-throughput labs.
Ready-to-Use Kit Contents
Each version includes all necessary components: a CD15 antibody cocktail, RapidSpheres™ magnetic particles, and 10X RBC lysis buffer. The RoboSep™ kit also contains disposable filter tips and additional buffer for seamless integration into automated systems.
Supporting Your Research
This CD15 positive selection kit suits a wide range of immunology and hematology applications. Whether you’re working on HLA studies or myeloid cell research, it provides the precision and consistency you need. Apex Scientific supports laboratories throughout South Africa with expert advice and fast delivery.
To learn more about immunomagnetic separation or explore additional tools from StemCell Technologies, visit our Insights page. You’ll find practical tips and product guidance tailored to South African scientists and their unique research environments.
