The CD66b positive selection kit from StemCell Technologies is available in South Africa through Apex Scientific, a trusted supplier of laboratory equipment. Designed for researchers working in immunology and chimerism, this kit isolates highly purified human CD66b+ granulocytes directly from whole blood using immunomagnetic positive selection. It combines targeted monoclonal antibodies with a column-free magnetic system, making the protocol both fast and efficient. With purities of up to 99%, this solution simplifies granulocyte enrichment for downstream analysis.
Efficient Granulocyte Isolation
The kit uses EasySep™ technology to label granulocytes with CD66b, specific antibody complexes and magnetic particles. These labeled cells are then retained by an EasySep™ magnet, allowing researchers to pour off the unwanted cells. The enriched CD66b+ population is immediately ready for use in flow cytometry, culture, or nucleic acid extraction. Since this process is column-free, it requires minimal laboratory equipment, only a magnet. Moreover, for higher sample volumes, the protocol is compatible with RoboSep™ automation, boosting throughput without compromising purity.
Key Benefits for South African Laboratories
This kit offers multiple advantages to scientists across South Africa. It enables:
- Rapid, user-friendly granulocyte isolation
- Up to 99% purity of CD66b+ cells
- A column-free workflow with fewer consumables
As a result, researchers save time and reduce equipment needs without sacrificing cell quality.
Kit Options and Components
Apex Scientific supplies two formats of the CD66b positive selection kit:
- EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit (Cat# 17882): Includes CD66b Selection Cocktail, Dextran RapidSpheres™, and 10× RBC Lysis Buffer.
- RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit (Cat# 17882RF): Adds RoboSep™ Buffer and Filter Tips for use with the RoboSep™ – S instrument.
Both versions come fully equipped with the reagents needed for efficient cell isolation.
Compatibility and Support
The kit is compatible with EasySep™ magnets, including the Big Easy and EasyEights™ options. It also integrates seamlessly with RoboSep™ – S for automation. In addition, Apex Scientific provides local support to help laboratories in South Africa adopt and optimize this solution.
To explore more about immunomagnetic separation and related workflows, visit the Apex Scientific Insights page.
