The CD14 positive selection kit II from StemCell Technologies enables fast, reliable isolation of human CD14+ monocytes using a column-free immunomagnetic process. Offered by Apex Scientific, this advanced kit delivers highly purified cells in just 20 to 30 minutes while preserving cell viability and function. Whether working with fresh or frozen PBMCs or leukapheresis samples, this kit integrates seamlessly into demanding laboratory workflows across South Africa.
Each kit includes ready-to-use monoclonal antibodies, magnetic particles, and buffers. As a result, scientists can accelerate sample preparation without compromising performance. Moreover, its streamlined design supports various downstream applications, including flow cytometry, macrophage culture, and nucleic acid extraction. Researchers in immunology, hematology, and cell therapy consistently choose this solution for its speed, reliability, and ease of use.
How It Works
The CD14 positive selection kit II applies EasySep™ immunomagnetic technology for efficient cell isolation. First, anti-CD14 monoclonal antibodies bind to target cells, and Dextran RapidSpheres™ attach to the antibody complexes. Next, the tube is placed in a compatible EasySep™ magnet. The magnetic field draws labeled cells to the side, while unbound cells are easily poured off. This quick, column-free method reduces manual handling and improves cell viability across protocols.
Key Features
- Rapidly isolates CD14+ monocytes in under 30 minutes
- Delivers up to 97% purity
- Eliminates columns and centrifugation steps
- Compatible with standard laboratory equipment
- Available in standard and large-scale kit formats
Applications
Scientists across South Africa use this kit for high-purity monocyte isolation, especially in immunology and translational research. It supports applications such as macrophage differentiation, functional assays, flow cytometry, and transcriptomics. In addition, Apex Scientific provides expert guidance, technical resources, and nationwide delivery to support your lab’s success.
To learn more about how this kit fits into your workflow, visit the Apex Scientific Insights page for in-depth scientific articles and product support.