The EasySep™ human monocyte enrichment kit by StemCell Technologies provides efficient laboratory isolation of highly purified human monocytes (CD14+CD16–) from blood samples. This immunomagnetic negative selection kit works seamlessly with fresh or frozen peripheral blood mononuclear cells (PBMCs) and lysed leukapheresis samples. In over 20 years of published research, EasySep™ has delivered reliable performance by combining monoclonal antibody specificity with a simple, column-free magnetic workflow. In fact, the EasySep™ human monocyte enrichment kit has been featured in dozens of scientific publications, underlining its strong performance and reputation in research laboratories. Furthermore, Apex Scientific, a leading supplier of laboratory equipment in South Africa, offers this kit so scientists nationwide can enrich monocytes with ease and consistency.
Isolation Procedure
This human monocyte enrichment kit uses antibody-conjugated magnetic particles to label and remove unwanted cells. Specifically, the procedure targets several cell types, including:
- Granulocytes
- T cells
- B cells
- Dendritic cells
- Natural killer (NK) cells
- Erythroid cells
When mixed with a PBMC sample, the magnetic particles bind these unwanted cells. The EasySep™ magnet then retains the labeled cells, leaving the untouched CD14+ monocytes in suspension. Finally, the researcher can simply pour or pipette the enriched monocytes into a new tube. The resulting monocyte fraction is highly pure and viable for downstream analysis, such as flow cytometry, cell culture, or DNA/RNA extraction.
Applications and Performance
After isolation, users obtain a monocyte fraction typically 83–95% pure (starting from frozen PBMCs). The isolated monocytes suit a variety of laboratory techniques, including functional assays and molecular analyses. For example, research groups in South Africa can integrate the human monocyte enrichment kit into immunology or vaccine development workflows, where pure monocyte populations are essential. Because the kit uses no separation columns, it speeds up processing and reduces handling steps. Moreover, all kit reagents and equipment are gentle on cells, preserving viability and function throughout the isolation.
Kit Variants and Related Products
StemCell Technologies also provides alternative kits for different project needs. For example, the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion omits removal of CD16+ monocytes for applications requiring that subset. Additionally, the EasySep™ Human Monocyte Isolation Kit (Catalog #19359) completes separations in about 12.5 minutes for even faster results. Apex Scientific equips customers with compatible equipment – such as EasySep™ magnets and automated RoboSep™ instruments – to streamline isolations. Furthermore, Apex Scientific offers related laboratory equipment, culture media, supplements, and antibodies to support monocyte culture and analysis.
To learn more about advanced cell isolation techniques and other lab equipment, visit the Apex Scientific Insights page for expert articles and guides.
