The Neutrophil isolation kit by StemCell Technologies is an advanced laboratory solution for quickly isolating highly purified human neutrophils via immunomagnetic negative selection. Now offered by Apex Scientific in South Africa, the Neutrophil Isolation Kit combines the specificity of targeted monoclonal antibodies with the simplicity of a column-free magnetic separation system. For over 20 years, researchers have trusted this EasySep™ technology to consistently yield high-purity neutrophils for immunology studies.
Immunomagnetic Negative Selection – How It Works
First, the kit’s specialized antibodies bind to unwanted white blood cells, tagging them with magnetic particles. Next, an EasySep™ magnet pulls these labeled cells to the side of the tube, so you can simply pour off or pipette out the untouched neutrophils into a fresh tube. As a result, the neutrophils remain unbound and highly pure. This simple, column-free procedure eliminates the need for additional separation equipment or centrifugation steps, streamlining your laboratory workflow with the Neutrophil Isolation Kit.
Rapid Isolation with High Purity and Viability
The entire isolation process using the Neutrophil Isolation Kit is complete in as little as 14 minutes, enabling a quick turnaround for your laboratory experiments. As soon as isolation finishes, the neutrophils are ready for downstream applications such as flow cytometry analysis, cell culture, or DNA/RNA extraction for molecular assays. Researchers typically achieve neutrophil purities of up to 99% with this isolation kit; consequently, subsequent analyses are more reliable and free of contamination by other cell types. Importantly, the neutrophils remain untouched and functionally viable, so you can use them immediately in sensitive laboratory assays.
Seamless Integration into Laboratory Workflows
This next-generation EasySep™ kit replaces the older EasySep™ Human Neutrophil Enrichment Kit (Catalog #19257), offering even faster cell isolations for busy laboratories worldwide. In particular, researchers in South Africa benefit from Apex Scientific’s distribution, which ensures ready access to this improved isolation kit, along with local support for seamless adoption in their laboratories. Additionally, the kit integrates seamlessly with existing laboratory workflows and is fully compatible with common sample preparation methods, including HetaSep™ sedimentation or red blood cell lysis for whole blood.
The Neutrophil isolation kit works with all EasySep™ magnets (from the standard EasySep Magnet to “The Big Easy” for larger volumes, EasyEights™ for multiple samples, and EasyPlate™ for 96-well formats). Furthermore, high-throughput labs can fully automate the process by using the RoboSep™ – S instrument with this kit. Because the procedure requires no columns or filtration steps, labs do not need any extra specialized equipment beyond a magnet and basic supplies. Thus, the neutrophil isolation kit reduces overall costs and complexity in the laboratory.
Kit Components Included
Apex Scientific supplies both versions of the isolation kit in South Africa to suit different laboratory setups:
- EasySep™ Human Neutrophil Isolation Kit (Catalog #17957) – Includes EasySep™ Human Neutrophil Isolation Cocktail (1 mL) and EasySep™ Dextran RapidSpheres™ (1 mL).
- RoboSep™ Human Neutrophil Isolation Kit with Filter Tips (Catalog #17957RF) – Includes EasySep™ Human Neutrophil Isolation Cocktail (1 mL), EasySep™ Dextran RapidSpheres™ (1 mL), RoboSep™ Buffer, and RoboSep™ Filter Tips.
Apex Scientific is committed to providing laboratories in South Africa with cutting-edge equipment like the neutrophil isolation kit, backed by local technical support and expertise. For expert tips and technical guidance on cell isolation and other laboratory techniques, visit the Apex Scientific Insights page for more resources.
