The human pan-B cells can be easily and efficiently isolated using the EasySep™ Human Pan-B Cell Enrichment Kit by StemCell Technologies, available through Apex Scientific in South Africa. This advanced laboratory equipment kit enables researchers to quickly purify human pan-B cells (including antibody-producing plasma cells) from human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. It uses an immunomagnetic negative selection process that removes unwanted cells while leaving the target human pan-B cells untouched and highly purified. The result is a fast, column-free method that delivers human pan-B cell populations of up to 99% purity for downstream analysis and applications in immunology research. Furthermore, for over 20 years EasySep’s immunomagnetic technology has been a trusted method for isolating human pan-B cells in laboratories worldwide, underscoring its reliability and strong track record in peer-reviewed research.
Efficient Immunomagnetic Separation of Human Pan-B Cells
In this kit’s negative selection procedure, specific monoclonal antibody cocktails and magnetic particles target non-B cells for removal. Unwanted cells bearing markers such as CD2, CD3, CD14, CD16, CD36, CD42b, CD56, CD66b, CD123, and glycophorin A bind to the magnetic particles, and the magnet then removes these labeled cells from the sample. Meanwhile, the desired human pan-B cells remain in the solution, untouched by any labels. Afterward, you simply pour or pipette the enriched B cell fraction into a new tube. As a result, the isolated B cells are immediately ready for downstream applications like flow cytometry, cell culture, or DNA/RNA extraction.
Key Advantages of the EasySep Kit
Fast and simple – The procedure is quick, easy to perform, and completely column-free, streamlining your laboratory workflow.
High purity – Achieve B cell purities up to 99%, ensuring you isolate a highly enriched population of human pan-B cells for accurate experimental results.
Untouched cells – No depletion antibodies or magnetic beads bind to the target human pan-B cells, so the purified cells remain truly untouched and functional for downstream assays.
Flexible Compatibility and Automation
The EasySep human pan-B cell enrichment kit works with a variety of EasySep™ magnets (from the standard EasySep Magnet to “The Big Easy” and Easy 50 magnets), allowing you to scale the procedure for different sample volumes. In addition, you can pair this kit with the RoboSep™-S instrument to fully automate the cell isolation process for higher throughput needs. The kit comes with a specialized antibody cocktail and magnetic particles for manual use. In South Africa, Apex Scientific also offers a RoboSep™-compatible version of this product (Catalog #19554RF) which includes the RoboSep buffer and disposable tips required for automated separation on the RoboSep-S platform. This flexibility lets you integrate the EasySep method seamlessly into different laboratory workflows, accommodating a range of throughput needs.
Applications and Special Considerations
Isolated human pan-B cells from this kit are ideal for a range of downstream applications in immunology. Researchers in South Africa and worldwide use these cells for phenotyping by flow cytometry, functional assays in laboratory research, antibody production studies, and more. For example, scientists can culture these untouched human pan-B cells to study B cell proliferation or stimulate them to differentiate into antibody-secreting plasma cells.
For instance, to isolate only CD43-negative B cells (excluding any B cell that might express CD43) from normal samples, StemCell Technologies offers the human B-Cell enrichment kit specifically for that purpose. Similarly, for samples from patients with B cell leukemia or lymphoma (where B cells may aberrantly express markers like CD43, CD36, or CD123), StemCell Technologies provides the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (Catalog #17963). Using this specialized kit ensures that even B cells with those markers remain in the final enriched fraction.
Apex Scientific offers full support and resources for scientists using this technology. You can find additional information and expert tips on our Insights page, which provides guidance on getting the most out of your laboratory equipment and reagents. As a leading provider of laboratory equipment in South Africa, we at Apex Scientific offer the EasySep Human Pan-B Cell Enrichment Kit as part of our portfolio of solutions to advance scientific discovery. Ultimately, we remain committed to empowering South African laboratories with reliable tools like this kit for cutting-edge work with human pan-B cells in immunology research.
