The T cell isolation kit by StemCell Technologies, available through Apex Scientific, enables laboratories in South Africa to rapidly isolate highly purified human T cells from blood samples. This isolation kit uses a fast immunomagnetic negative selection process that combines specific monoclonal antibodies with a simple, column-free magnetic system for efficient T cell enrichment. Moreover, this kit is part of Apex Scientific’s advanced laboratory equipment offerings and is tailored to meet the needs of scientific research settings.
Immunomagnetic Negative Selection Workflow
In this workflow, the kit labels unwanted cells with targeted antibody complexes and magnetic particles. A dedicated EasySep™ magnet then pulls out these tagged cells, removing any cell expressing unwanted markers (for example, CD14, CD16, CD19, CD36, CD56, or GlyA) from the sample. As a result, the remaining population is a highly purified collection of untouched T cells, ready for use. The entire isolation procedure takes as little as 8 minutes, and thus researchers can move quickly to downstream applications like flow cytometry analysis, cell culture experiments, or DNA/RNA extraction. Furthermore, because the method uses negative selection, the desired T cells remain unbound by labels – preserving their functionality for sensitive immunology assays.
Key Advantages
For scientists in South Africa, this human T cell isolation kit provides several key advantages:
- Fast, easy, and column-free: The immunomagnetic separation is straightforward, and you can complete it in minutes without cumbersome columns or complicated steps, making it ideal for busy laboratory workflows.
- High purity and recovery: Achieve up to 98% T cell purity with high cell recovery rates, ensuring that research labs in South Africa get maximum yield of target cells from each sample.
- Untouched, viable cells: The isolated T cells remain untouched by any labeling or modification, resulting in a highly viable cell population that is immediately ready for downstream use.
Applications in Research
This T cell isolation kit supports a wide range of immunology and cell therapy applications. Researchers can immediately use the purified T cells in downstream assays — for example, immunophenotyping by flow cytometry, expansion in culture, or other molecular analyses. This kit has proven to be a trusted tool in laboratories for over 20 years, and it continues to help scientists across South Africa advance their research with confidence.
Compatibility and Automation
The EasySep™ human T cell isolation kit is compatible with all EasySep™ magnets and automation systems used in the laboratory. It works with the standard EasySep magnet for small samples, larger magnets like “The Big Easy”, Easy 50, EasyPlate™, EasyEights™, and Easy 250 for higher-volume processing, as well as the RoboSep™ – S instrument for fully automated cell isolation. In this way, you can easily scale up or automate T cell isolation as needed, making this kit a versatile piece of equipment for any immunology laboratory. For even larger sample requirements, a large-format kit is available.
Learn More
To explore further or get technical tips on cell isolation, visit our Apex Scientific Insights page for expert resources. Our team at Apex Scientific is here to help you choose the right laboratory equipment for your research needs across South Africa.
