Gentle Cell Dissociation Reagent (GCDR) is an enzyme-free, chemically defined solution for dissociating human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells into small aggregates or single-cell suspensions for routine passaging in cell culture. In addition, this gentle reagent supports high cell viability and expansion of pluripotent stem cell colonies during laboratory culture. It avoids harsh enzymes, helping sensitive stem cells remain healthy so they can continue expanding efficiently.
Versatile Applications in Organoid Culture
Additionally, the gentle cell dissociation reagent is versatile for other laboratory applications beyond pluripotent stem cell maintenance. For instance, researchers use this reagent to isolate intestinal crypts from human or mouse tissues and to gently break up Corning® Matrigel® domes when passaging 3D organoid cultures. As a result, delicate intestinal and epithelial organoids can be transferred or expanded with minimal cell damage, improving the success of organoid experiments in the laboratory.
cGMP-Grade Quality and Consistency
Furthermore, StemCell Technologies now manufactures the dissociation reagent under stringent current Good Manufacturing Practices (cGMP) in a certified quality-controlled facility. Consequently, each batch of the gentle cell dissociation reagent offers consistent performance and reliable results for reproducible experiments. The chemically defined formulation also eliminates variability, ensuring that your laboratory workflows remain consistent over time.
Key Advantages of Gentle Cell Dissociation Reagent
- Gentle, enzyme-free formulation: Protects cells during dissociation with a chemically defined, non-enzymatic solution (manufactured under GMP for quality), resulting in healthy, high-quality cultures.
- High expansion yields: Enables robust expansion of human ES and iPS cell cultures during routine laboratory passaging, maintaining pluripotent colonies without compromising cell health.
- Easy room-temperature protocols: Simplifies cell passaging with straightforward procedures at ambient temperature, with no need for specialized equipment or complicated steps.
Supporting Laboratories in South Africa
Apex Scientific is the leading laboratory equipment supplier in South Africa, making advanced products like the cell dissociation reagent readily accessible to local researchers. Moreover, our team provides expert technical support and service throughout South Africa to ensure you get the best results with gentle cell dissociation reagent in your laboratory workflows. Additionally, visit the Apex Scientific Insights page for expert tips, protocols, and the latest industry trends in laboratory science.
