The ventricular cardiomyocytes derived from human pluripotent stem cells are essential for cardiac research and drug discovery. The STEMdiff Ventricular Cardiomyocyte Differentiation Kit from StemCell Technologies provides serum-free media to differentiate human ES or iPS cells into beating ventricular heart muscle cells, along with maintenance medium to support long-term culture. This simple, serum-free monolayer protocol produces a densely packed sheet of contracting cardiomyocytes in about 15 days. Over 80% of these cells express cardiac troponin T (cTnT), and labs typically harvest ~1×10^6 cells per well of a 12-well plate. Additionally, Apex Scientific is a trusted supplier of laboratory equipment in South Africa, helping local labs obtain the tools needed for large-scale cardiomyocyte cultures.
Key Advantages
- Complete workflow – Straightforward, serum-free protocol covers the full hPSC-to-cardiomyocyte differentiation process, yielding beating cells in ~15 days.
- High yield and purity – One kit generates >50 million cTnT-positive cardiomyocytes; differentiated cultures routinely exceed 80% cardiomyocyte purity.
- Consistent, robust performance – Optimized for various human pluripotent stem cell lines, the kit delivers reliable results with minimal line-to-line variability.
- Long-term maintenance – Includes specialized basal medium and supplement to maintain hPSC-derived cardiomyocytes for 30+ days.
- Local support – Apex Scientific provides local expertise, training, and guidance to help South African laboratories implement the protocol effectively.
Kit Contents
- Cardiomyocyte Differentiation Basal Medium (380 mL)
- Ventricular Cardiomyocyte Differentiation Supplements A & B (10X, 10 mL each) and C (10X, 20 mL)
- Cardiomyocyte Maintenance Basal Medium (490 mL)
- Cardiomyocyte Maintenance Supplement (50X, 10 mL)
Applications
For example, these high-quality ventricular cardiomyocytes are ideal for in vitro laboratory research, including cardiac disease modeling, drug screening, and toxicity testing. Furthermore, Apex Scientific provides this specialized laboratory equipment to support laboratories and researchers in South Africa, helping scientists produce consistent and reliable cell cultures. Apex Scientific also offers technical support and training so that laboratories across South Africa can efficiently implement this workflow. For more information and technical articles, visit the Apex Scientific Insights page.
