The Cerebral organoid maturation kit is a specialized serum-free media solution from StemCell Technologies that enables extended culture of human PSC-derived neural organoids beyond 40 days. This kit includes media optimized to mature cerebral organoids into multi-region, brain-like structures in the laboratory. Additionally, the maturation kit integrates seamlessly with the STEMdiff™ Cerebral Organoid Kit, allowing researchers to first generate organoids under defined, serum-free conditions and then continue their maturation.
Key Features and Advantages
Key features of the cerebral organoid maturation kit includes:
- Multi-region organoids: This maturation kit’s formula supports the formation of unpatterned cerebral organoids that spontaneously differentiate into multiple brain regions within the same organoid. This allows researchers to model complex neural tissue architecture in a single organoid.
- Flexible culture formats: With this maturation kit, organoids can be matured either embedded in a matrix droplet or cultured in a free-floating liquid suspension. This flexibility lets scientists adapt protocols for different laboratory workflows or imaging techniques.
- Efficient organoid formation: The media are based on a published protocol, increasing the efficiency and consistency of organoid maturation. As a result, labs can achieve higher yields of functional organoids using this optimized approach.
- Expandable platform: Because this maturation kit is highly compatible with existing STEMdiff organoid systems, researchers can easily develop new or modified organoid models for various research applications. This compatibility simplifies the creation of custom disease models or experimental workflows.
Applications
The STEMdiff™ cerebral organoid maturation kit is ideal for disease modeling, neuroscience, and stem cell research. In fact, many laboratories in South Africa supplied by Apex Scientific use this kit to model neural development and disease in vitro. In practice, scientists use mature organoids from this kit for downstream analyses such as functional assays and immunofluorescence imaging. For example, organoids cultured over 40 days provide a valuable in vitro model for studying later stages of neural development, synapse formation, and disease pathology.
Kit Contents
- STEMdiff™ Cerebral Organoid Basal Medium 2 (250 mL) – a defined serum-free basal medium optimized for cerebral organoid culture.
- STEMdiff™ Cerebral Organoid Supplement E (4.5 mL) – a specialized supplement that provides key nutrients and factors needed during extended organoid maturation.
Why Apex Scientific
Apex Scientific is dedicated to providing laboratories in South Africa with world-class scientific solutions. As a trusted laboratory equipment supplier, we support scientists with nationwide service, equipment installation, and training. Moreover, our local technical team can assist with STEMdiff cerebral organoid protocols to ensure reliable results.
Additionally, Apex Scientific supplies the STEMdiff™ cerebral organoid maturation kit in South Africa and offers local support for its implementation. In fact, researchers using the cerebral organoid maturation kit can find additional tips on our Apex Scientific Insights page.
