The Biomolecular Sapphire FL/+ from Azure Biosystems delivers high-resolution, laser-based imaging for a variety of laboratory applications. With point-by-point laser scanning, this benchtop imager achieves ultra-high resolution (5–1,000 μm) and supports fluorescent Western blots, gel imaging, slide-based tissue analysis, and even live animal imaging. Additionally, Apex Scientific supplies the Sapphire FL in South Africa, providing local support, training, and service to ensure optimal performance.
Key Features and Technology
The Sapphire FL/+ uses point-scanning laser optics, focusing all excitation light on a single point. Consequently, it achieves exceptional signal-to-noise and high sensitivity. Moreover, PMT/APD detectors capture fluorescence and phosphor signals, while an optional sCMOS camera handles chemiluminescence. Its wavelength range spans 375–900 nm, covering UV to near-infrared dyes. Furthermore, extended dynamic range (EDR) mode captures both weak and strong signals simultaneously, preserving linearity for quantitative assays.
Modular Laser and Filter Design
Researchers can easily swap laser modules and emission filters within minutes. Standard modules cover 375, 450, 488, 532, 638, 658, 685, 730, and 784 nm, along with a 400–606 nm band module for phosphor screens. This flexibility allows labs to optimize imaging for multiple dyes or chemistries. As a result, they are not limited to factory presets and can adapt the system as experimental needs evolve.
Multi-Mode Imaging
The Biomolecular imagers support fluorescence, chemiluminescence, and phosphor imaging on a single platform. For instance:
- Fluorescence: Capture up to four channels simultaneously for multiplex assays.
- Chemiluminescence: An optional module detects transient low-light signals for Western blots and other assays.
- Phosphor Imaging: Scans radio-labeled screens with high sensitivity and linearity over 5+ orders of magnitude.
These modes enable imaging of Western blots, gels, slides, plates, tissues, and live small animals—all with one instrument.
Laboratory Applications
For example, in Western blots, researchers can multiplex up to four fluorescent targets without stripping blots, while quantitative measurements remain accurate across weak and strong bands. Similarly, gel imaging produces high-resolution images with uniform illumination. Slide and tissue scanning captures microarrays or tissue sections at up to 5 μm resolution. In addition, in vivo imaging benefits from five built-in anesthesia ports and a 4 cm imaging window, allowing longitudinal studies in live mice.
Choosing the Right Biomolecular Imager
When selecting an imager, labs should consider sensitivity, wavelength coverage, dynamic range, modularity, throughput, and accessory availability. The Sapphire FL provides all these features. In addition, optional plate and slide holders enhance high-throughput workflows, giving researchers greater flexibility in experimental design.
Why Apex Scientific and Azure Biosystems
As the exclusive South African supplier for Azure Biosystems, Apex Scientific ensures that local customers receive tailored support, training, and service. Consequently, laboratories can rely on consistent performance and efficient integration of the Sapphire FL into their research workflows.
Overall, the Sapphire FL/+ combines high resolution, multi-mode detection, and flexible sample handling. Therefore, it is ideal for labs performing Western blots, gel imaging, slide scans, and live-animal studies. For South African researchers seeking versatile and sensitive imaging solutions, the Sapphire FL represents a comprehensive, all-in-one option.
