UV/Vis Absorbance
Wavelength selection in Feyond-A300 is done by using an advanced monochromator system. Any wavelength between 200 to 1000 nm can be selected. Using the spectral scanning feature, the whole spectrum of a sample can be scanned in 1 nm increments to allow identification of the optimal measurement wavelength for a new assay.
Fluroescence
Feyond-A300 is equipped with filter-based fluorescence optics and dichroic mirrors for screening applications such as fluorescence polarization, and TR-FRET. Standard applications such as fluorescence-based DNA/RNA quantification assays are not only supported in microplates, but also in low volume u-Nano Ultra-Micro plate. The independent removable filter modules make it more convenient for users to replace the filter. The filter-based fluorescence optics detection ensures high sensitivity, greater light transmission, precise control over transmitted peak shape, excellent
blockage of undesired wavelengths. This is ideal for excitation and emission applications. The filters are also the technically preferred and most cost efficient technology for non-absorbance based assays.
Luminescence
Feyond-A300 has been designed with a dedicated luminescence detection system for both flash and glow based assays. It can offer excellent sensitivity and wide dynamic range in luminescence measurement. The optimized precision dual-channel injectors ensure the detection performance of the 96-well and 384-well plate. The PMT enhances the maximum sensitivity of weak luminescence signals, prevents over saturation of high signals, effectively improves the detection range of luminescence and minimizes signal cross talk between wells.
Time-Resolved Fluorescence
Fluroescence Polarization
Software
User Authority Classification
- User permissions are divided into four levels, with clear permissions
- The users have independent accounts and passwords to ensure the safety and confidentiality of experimental results.
Intuitive Interface Display
- Intuitive selection of function mode, easy parameter setting
- Programs and results are stored independently, making it easy to find the required applications.
Powerful Data Analysis and Process
- Provide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis.
- Algorithm customization: according to your assays needs, can customize the required algorithm.
Liberalized User Communication
- The software has a shared library, which can store the program, results, and standard curves for sharing them with others
- The program and standard curve can be created in real time to a QR code, and the required content can be imported only by scanning the code with the instrument.
File Transfer Protocol
- Upload the data directly to a computer with a FTP server, and users can view the data results at any time in the authorized folder
Technical Specifications
| Model | Feyond |
| Absorbance | |
| Light source | Xenon lamp |
| Detector | PD |
| Wavelength accuracy | 2 nm |
| Wavelength repeatability (SD) | 0.2 nm |
| Half width (FWHM) | <2.5 nm |
| Wavelength range | 200-1000 nm, 1 nm step |
| Measuring range | 0-4 OD |
| Resolution | 0.0001 OD |
| Accuracy@450nm | 96-precision mode: ± (1.0 %+0.003 Abs) @(0.0-2.0 Abs] ±2.0 % @(2.0-3.0 Abs] |
| Repeatability | CV < 1.0 % or SD < 0.003 fast (0.0-3.0Abs] CV < 0.5 % or SD < 0.003 accurate (0.0-3.0Abs] |
| Stray light | 0.1 % @220 nm |
| Linear@450nm | R²≥0.999@[0.0-3.0Abs] |
| Reading time | 96-well plate: fast <15 s |
| Luminescence | |
| Detector | PMT |
| Detection limit | 15 amol/hole; 5 amol/hole (photon PMT) |
| Linear dynamic range | 6 logs |
| Crosstak | ≤0.005% |
| Wavelength range | 200-850 nm |
| Fluorescence | |
| Reading mode | Top reading |
| Excitation light source | Xenon lamp |
| Detector | PMT |
| Wavelength range | EX:200-1000 nm;EM:270-850 nm |
| Filter EX/EM | 3 groups: EX470/EM525, EX523/EM564, EX624/EM692 (other wavelengths can be replaced) |
| Detection limit | 1:00 PM |
| Linear dynamic range | 6 logs |
| TRF | |
| Wavelength range | EX:200-1000 nm; EM:270-850 nm (Feyond-A400/Feyond-A500) |
| Detection limit | 0.05 pM (Feyond-A400/Feyond-A500) |
| FP | |
| Wavelength range | 300-850 nm (Feyond-A500) |
| Detection limit | 5mP (Feyond-A500) |
