Category Archives: Handheld Spectrophotometers

Turbidity measurement of unknown liquid samples using Photopette

Determining Turbidity of an aqueous solution is a frequently used procedure to estimate the quality of water, or even concentration of suspended particles such as cells, bacteria and mud.Turbidity can be defined as a decreased in the transparency of a solution due to the presence of suspended and some dissolved substances, which causes incident light […]

On site Nickel Measurement for the Metal Plating Industry

A method for on-site nickel measurements for the metal plating industry is introduced. By using the Photopette® handheld spectrophotometer [1, 2] only minimal training is requited. The method is easy and fast performed in two steps 1) Calibration and 2) Measurement. The calibration takes about 3 minutes and is stable for several days. A measurement […]

Determination of Biomass in Spirulina cultures by Photometry

Spirulina is a microscopic and filamentous cyanobacterium. It is promoted as a ‘superfood’ that contribute to high energy levels. The important nutrients are polysaccharides and essential fats which are easily absorbed by human cells and help in energy release [1]. Generally, the growth of the bacteria is characterized by five stages. To maintain a healthy […]

Determination of Ethanol in Alcoholic Beverages Using Photopette

The food & beverage industry must make many tests and take certain measurements to ensure that their products meet high standards and are safe for consumption. The Photopette can be used for photometric measurements of liquid samples. The objective of this application note is to demonstrate how Photopette Cell can be used to quantify the […]

E. coli Cell Count at OD600

E.coli cell counting at OD600 is demonstrated using the Photopette® handheld spectrophotometer [1, 2]. Almost no training is requited. The method is easy and fast performed at the bioreactor or in the cell culture hood. E. coli can be directly measured at 600 nm in the cell culture flask. The method can be performed at […]

Yeast Cell Count At OD600

Yeast cell counting at OD600 is demonstrated using the Photopette® handheld spectrophotometer [1, 2]. Almost no training is requited. The method is easy and fast performed at the brewery or in the cell culture hood. Yeast can be directly measured at 600 nm in the cell culture flask. The method can be performed at any […]

Measuring Cell-Viability By Resazurin (AlamarBlue®) Assay Using Photopette® Cell

The objective of this application note is to demonstrate how Photopette Cell can be used to measure cell-viability of eukaryotic cells using a Resazurin Assay. Furthermore, it acts as a handy guide to get you started with Photopette Cell, and outlines application-specific parameters for reference. Cell-viability assays are important cell-based assays to determine whether cells […]

FAST PANTOPRAZOLE MEASUREMENT IN PILLS

Here we demonstrate a method to measure the pantoprazolecontent in tablets within 10 minutes. There are no reagentsnecessary. By using the Photopette® handheldspectrophotometer [1, 2] only minimal training is requited.The method is easy and fast performed in two steps 1)Calibration and 2) Measurement. The method can beperformed at any location and does not require a […]

DETERMINATION OF LACTATE CONCENTRATION USING PHOTOPETTE CELL

During anaerobic glycolysis, energy is produced. In this process, lactate dehydrogenase (LDH) catalyses the oxidation of lactate to pyruvate and an equimolar amount of nicotinamide adenine dinucleotide (NAD+) is reduced to its reduced form NADH. The amount of NADH produced is directly proportional to the lactate concentration in the sample [1]. The produced NADH is […]

MEASURING PROTEIN CONCENTRATION DIRECTLY USING PHOTOPETTE® BIO

Download PDF version of this article here Measuring Protein Concentration Directly using Photopette® Bio Photopette® enables fast protein measurements directly at the bench.Protein samples can be directly measured at 280 nm without sample preparation and sample transfer. Objective: The objective of this application note is to demonstrate how the Photopette® Bio device can directly measure […]

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