In order to maintain cellular viability during the freezing process, certain cells – such as protist, mammalian, and plant cells – require precise control of the freezing rate in order to minimize the detrimental effects of undercooling and the heat liberated during the phase change process from water to ice. Controlled rate freezing is an established procedure for the cryopreservation of biologic material in research and clinical applications. In-line with GMP protocols, cell material can be frozen then stored at cryogenic temperatures.CryoVita controlled-rate freezer (CRF) achieve reliable, reproducible results with maximum operational safety. They are used for clinical, veterinary and research tasks for controlled rate freezing of samples.
Dependable sample protection
For customers in research, cell and gene therapy, vaccine production, and biobanking, the CryoVita CRF provides precise, repeatable freezing results that protect the sample from intracellular freezing. Liquid nitrogen flows via a metal hose connection, as a gas, into the freezing chamber. Predetermined cooling profiles are used to freeze samples prior to removal and storage at cryogenic temperatures. Data logging ensures continual monitoring of the entire process, including multiple temperature sensor set-points, actual values and the supply pressure of liquid nitrogen.
Reliable temperature performance for high-throughput usage
- Dual solenoid valves design, one for working, the other for backup. When one valve fails, switch to the other valve through touch screen controller
- Consistent temperature control and uniformity achieved via an air-handling system and liquid nitrogen injection device
- Environmentally friendly insulating foam
- Type 304 stainless steel with exterior powder-coat finish
User-friendly operation with enhanced data traceability
- Intuitive touchscreen display allows for easy set-up, operation, and review of a freezing run
- Six pre-set freezing profiles and space for up to 120 user-defined, “custom” freeze profiles
- Convenient to create and edit a custom program on touch screen
- Record temperature curve of each cycle automatically
- External influences are avoided during freezing
Real-time run monitoring for sample protection
- Chamber and sample temperatures are monitored by Type T thermocouples, eliminating lag time and providing “real time” responsiveness
- Standard alarms to alert users of thermocouple failures, heater malfunction, high/low temperature limits, temperature tracking, power failure, and completion of run
- RS232 Port
- USB port to download running data
Installation
- CryoCenter series self-pressurized liquid nitrogen
- Container is recommended to supply liquid nitrogen
- CryoVita series controlled rate freezers.
- 1.0.1MPa pressure
- Pressure building regulator
- Liquid level meter (float type/ electric digital type)
- Casters for easy movement
Technical Specifications
| Model | CryoVita 18 | CryoVita 34 | CryoVita 49 |
| Door type | Front open (upright) | Front open (upright) | Front open (upright) |
| Capacity (Liter) | 18 | 34 | 49 |
| 2ml vial | 380 | 805 | 1185 |
| 5ml vial | 228 | 483 | 711 |
| 25/50ml blood bag | 30 | 60 | 90 |
| 250ml blood bag | 10 | 20 | 30 |
| 500ml blood bag | 8 | 16 | 24 |
| 1/2 cc straw | 330 | 660 | 990 |
| Interior dimensions W x D x H (mm) |
180 x 305 x 330 | 330 x 305 x 330 | 485 x 305 x 330 |
| Exterior dimensions W x D x H (mm) |
810 x 540 x 790 | 960 x 540 x 790 | 1110 x 540 x 790 |
| Net weight (kg) | 64 | 75 | 83 |
| Heating power (W) | 2500 | 3000 | 3000 |
| Power supply | AC 100~240V, 50/60Hz | ||
| Interior material | SUS304 | ||
| Door seal | Yes | ||
| Temperature range (°C) | -180 ~ 50 | ||
| Freezing rate (°C/min) | 0.1 ~ 60 | ||
| Heating rate (°C/min) | 0.1 ~ 10 | ||
| Temperature deviation (°C) | <2 | ||
| Controller | Touch screen & microprocessor | ||
| Freezing program storage | Up to 20 (6 default programs) | ||
| Data storage and USB port | Yes | ||
| Chamber temperature display | Yes | ||
| Sample sensor & temp display | Yes | ||
| Freezing step display | Yes | ||
| Running time display | Yes | ||
| Remaining time display | Yes | ||
| Door-operation safety interlock | Yes | ||
| Refrigerant | Liquid nitrogen | ||
| Prefilter for liquid nitrogen | Yes | ||
| Liquid nitrogen source pressure | 14 ~ 22 PSI | ||
| Pressure relief valve | Yes, 125 PSI | ||
| Freezing racks | Optional | ||
| Model | CryoVita 26H | CryoVita 90H | CryoVita 150H |
| Door type | Top open (horizontal) | Top open (horizontal) | Top open (horizontal) |
| Capacity (Liter) | 26 | 90 | 150 |
| 0.25ml Straw | 900 | 2700 | 4500 |
| 0.5ml Straw | 630 | 1890 | 3150 |
| Interior dimensions W x D x H (mm) |
476 × 184 × 340 | 476 × 552 × 340 | 476 × 767 × 380 |
| Exterior dimensions W x D x H (mm) |
701× 769× 539 | 701× 1244 × 570 | 711× 1444 × 584 |
| Net weight (kg) | 40 | 62 | 140 |
| Heating power (W) | 2500 | 3000 | 3000 |
| Power supply | AC 100~240V, 50/60Hz | ||
| Interior material | SUS304 | ||
| Door seal | Yes | ||
| Temperature range (°C) | -180 ~ 50 | ||
| Freezing rate (°C/min) | 0.1 ~ 50 | ||
| Heating rate (°C/min) | 0.1 ~ 10 | ||
| Temperature deviation (°C) | <2 | ||
| Controller | Touch screen & microprocessor | ||
| Freezing program storage | Up to 20 (6 default programs) | ||
| Data storage and USB port | Yes | ||
| Chamber temperature display | Yes | ||
| Sample sensor & temp display | Yes | ||
| Freezing step display | Yes | ||
| Running time display | Yes | ||
| Remaining time display | Yes | ||
| Door-operation safety interlock | Yes | ||
| Refrigerant | Liquid nitrogen | ||
| Prefilter for liquid nitrogen | Yes | ||
| Liquid nitrogen source pressure | 14 ~ 22 PSI | ||
| Pressure relief valve | Yes, 125 PSI | ||
| Freezing racks | Optional | ||
