KEY FEATURES & BENEFITS:
– Less than 25 sec to get results
– Cell therapy quality control
– Cells countable with ADAM-MC2: stem cell / CAR-T cell / CAR-NK cell / Adipose-derived stem cell
– Automated image analysis
– Accurate result, sensitve CMOS detection, and precise automatic stage
A Fast & Accurate Nucleus Cell Counter
ADAM-MC2 is a new standard of automated fluorescence cell counter.
ADAM stands for Advanced Detection and Accurate Measurement.
– Less than 25 sec to get results
– Cell therapy quality control
– Cells countable with ADAM-MC2: stem cell/ CAR-T cell/ CAR-NK cell/ Adipose-derived stem cell
– Automated image analysis
– Accurate result with PI staining method
– Sensitive CMOS detection, and precise automatic stage
Advantage 1: Principle of Viability Measurement (PI-Staining Method)
After the samples are stained with fluorescent dye, propidium iodide, which intercalates DNA to stain the nucleus of target cells,
ADAM-MC2 takes fluorescent images automatically.
The obtained images are processed by image analysis software integrated inside the system.
Advantage 2: Counting Aggregated and Irregular-Shaped Cells
ADAM-MC2 provides accurate and reliable results because it counts aggregated and irregular-shaped cells.
In the image above, the images on the right side indicate cells that have been counted by ADAM-MC2.
– Accurate count based on cell size and shape
– Count aggregated cells individually
– Debris is excluded from results
Advantage 3: Cell Therapeutic Applications
ADAM-MC2 can be used as a device to monitor and QC the cell numbers and viability
in the process of manufacturing cells (CAR-T cells, stem cells, etc.) for cell therapy.
In addition, It is possible to use ADAM-MC2 depending on the cell types (Whole blood cell, PBMCs, etc.)
that need to be monitored during the manufacturing of cell therapy products.
Advantage 4: Accuracy & Reproducibility
The graph on the left shows correlation of total cell counting between hemocytometer and ADAM-MC2 using SH-SY5Y cells.
The graph on the right shows that samples with low, medium and high concentration of cells were counted with ADAM-MC2.
The repeatability at each level of cell concentration is high.
Advantage 5: Comparison of Cell Viability
Comparison of cell viability between ADAM-MC2, flow cytometry, and manual counting
SH-SY5Y, Jurkat, HeLa cells were treated with 100, 300 μM H2O2 for 3 hours,
then analyzed by ADAM-MC2, flow cytometry, and manual counting.
| Product name / Cat. No. | ADAM-MC2 / ADAM-MC2 |
| Loading volume | 23 µL/test (2ch), 13 µL/test (4ch) |
| Measuring volume | 8.6 µL/test (2ch), 3.4 µL/test (4ch) |
| Measurement range | 5 X 10E4 ~ 4 X 10E6 cells/mL |
| Focus | Auto-focusing |
| LED | 4W Green LED |
| Weight | 7.0 kg |
| Size (LxWxH) | 277 x 276 x 270 mm |
| Analysis time | < 50 sec/test (2channel),
< 25 sec/test (4channel) |
| Dyes | PI (Propidium Iodide) |
| Connectivity | WIFI (USB Dongle) |
| Cell type | Cell line (clumpy cell, single cell), PBMCs, Adipose Stem cell, Total white blood cell, Primary cells, SVF-derived cells |
1. Polymorphic Region-Specific Antibody for Evaluation of Affinity-Associated Profile of Chimeric Antigen Receptor, Molecular Therapy Oncolytics, 17, pp. 293-305, 2020. Link: https://www.sciencedirect.com/science/article/pii/S2372770520300541
